CACYBP Polyclonal Antibody, Cat#CAC13490
Western Blot, Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, HUVEC whole cell lysate, All lanes: CACYBP antibody at 2.7ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 27, 9, 22 kDa, Observed band size: 27 kDa
Immunohistochemistry of paraffin-embedded human lung cancer using CAC13490 at dilution of 1:100
Immunofluorescence staining of U251 cells with CAC13490 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating CACYBP in Hela whole cell lysate, Lane 1: Rabbit control IgG instead of CAC13490 in Hela whole cell lysate., For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC13490 (8ug) + Hela whole cell lysate (500ug), Lane 3: Hela whole cell lysate (10ug)
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CACYBP Polyclonal Antibody, Cat#CAC13490 Western Blot, Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, HUVEC whole cell lysate, All lanes: CACYBP antibody at 2.7ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 27, 9, 22 kDa, Observed band size: 27 kDa Immunohistochemistry of paraffin-embedded human lung cancer using CAC13490 at dilution of 1:100 Immunofluorescence staining of U251 cells with CAC13490 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunoprecipitating CACYBP in Hela whole cell lysate, Lane 1: Rabbit control IgG instead of CAC13490 in Hela whole cell lysate., For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC13490 (8ug) + Hela whole cell lysate (500ug), Lane 3: Hela whole cell lysate (10ug)

CACYBP Polyclonal Antibody

BiomatikSKU: CAC13490-100ug
Size: 100ug
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Western Blot, Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, HUVEC whole cell lysate, All lanes: CACYBP antibody at 2.7ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 27, 9, 22 kDa, Observed band size: 27 kDa
Immunohistochemistry of paraffin-embedded human lung cancer using CAC13490 at dilution of 1:100
Immunofluorescence staining of U251 cells with CAC13490 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating CACYBP in Hela whole cell lysate, Lane 1: Rabbit control IgG instead of CAC13490 in Hela whole cell lysate., For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC13490 (8ug) + Hela whole cell lysate (500ug), Lane 3: Hela whole cell lysate (10ug)

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