Ultra Sensitive IHC

Ca2 Polyclonal Antibody

BiomatikSKU: CAC07295-100ug

Size: 100ug
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Description

Technical Documents

Images

Western Blot; Positive WB detected in: Rat kidney tissue, Rat heart tissue, Rat liver tissue, Mouse lung tissue, Mouse kidney tissue; All lanes: CA2 antibody at 2µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 30 kDa; Observed band size: 30 kDa
Immunohistochemistry of paraffin-embedded human kidney tissue using CAC07295 at dilution of 1:100
Immunofluorescent analysis of Hela cells using CAC07295 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
IHC image diluted at 1:400 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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