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Immunoprecipitating C16orf87 in HepG2 whole cell lysate; Lane 1: Rabbit control IgG instead of CAC09423 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: CAC09423 (8µg) + HepG2 whole cell lysate (500µg); Lane 3: HepG2 whole cell lysate (10µg)
Western blot; ,All lanes: C16orf87 antibody at 3µg/ml + HepG2 whole cell lysate; ,Secondary; ,Goat polyclonal to rabbit IgG at 1/10000 dilution; ,Predicted band size: 18, 11 kDa; ,Observed band size: 18 kDa

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