Butyrly-Hist1H4A (K16) Polyclonal Antibody, Cat#CAC11432
Western Blot; Detected samples: 293 whole cell lysate, K562 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h; All lanes: HIST1H4A antibody at 1:100; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 12 kDa; Observed band size: 12 kDa
Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CAC11432 at 1:5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H4A (CAC11432) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ß-Globin promoter.
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Butyrly-Hist1H4A (K16) Polyclonal Antibody, Cat#CAC11432 Western Blot; Detected samples: 293 whole cell lysate, K562 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h; All lanes: HIST1H4A antibody at 1:100; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 12 kDa; Observed band size: 12 kDa Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CAC11432 at 1:5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H4A (CAC11432) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ß-Globin promoter.

Butyrly-Hist1H4A (K16) Polyclonal Antibody

BiomatikSKU: CAC11432-100ul
Size: 100ul
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Western Blot; Detected samples: 293 whole cell lysate, K562 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h; All lanes: HIST1H4A antibody at 1:100; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 12 kDa; Observed band size: 12 kDa
Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CAC11432 at 1:5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H4A (CAC11432) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ß-Globin promoter.

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