Butyrly-Hist1H4A (K12) Polyclonal Antibody, Cat#CAC11484
Western Blot; Detected samples: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h; All lanes: HIST1H4A antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/40000 dilution; Predicted band size: 12 kDa; Observed band size: 12 kDa
Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CAC11484 at 1:75, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating HIST1H4A in Hela whole cell lysate; , Lane 1: Rabbit control IgG instead in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: CAC11484 (3µg) + Hela whole cell lysate (500µg); , Lane 3: Hela whole cell lysate (20µg)
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Butyrly-Hist1H4A (K12) Polyclonal Antibody, Cat#CAC11484 Western Blot; Detected samples: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h; All lanes: HIST1H4A antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/40000 dilution; Predicted band size: 12 kDa; Observed band size: 12 kDa Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CAC11484 at 1:75, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunoprecipitating HIST1H4A in Hela whole cell lysate; , Lane 1: Rabbit control IgG instead in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: CAC11484 (3µg) + Hela whole cell lysate (500µg); , Lane 3: Hela whole cell lysate (20µg)

Butyrly-Hist1H4A (K12) Polyclonal Antibody

BiomatikSKU: CAC11484-100ul
Size: 100ul
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Western Blot; Detected samples: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h; All lanes: HIST1H4A antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/40000 dilution; Predicted band size: 12 kDa; Observed band size: 12 kDa
Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CAC11484 at 1:75, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating HIST1H4A in Hela whole cell lysate; , Lane 1: Rabbit control IgG instead in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: CAC11484 (3µg) + Hela whole cell lysate (500µg); , Lane 3: Hela whole cell lysate (20µg)

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