Butyrly-Hist1H3A (K18) Polyclonal Antibody, Cat#CAC11535
Western Blot; Detected samples: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h; All lanes: HIST1H3A antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/40000 dilution; Predicted band size: 16 kDa; Observed band size: 16 kDa
Immunocytochemistry analysis diluted at 1:200 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Butyrly-Hist1H3A (K18) Polyclonal Antibody, Cat#CAC11535 Western Blot; Detected samples: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h; All lanes: HIST1H3A antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/40000 dilution; Predicted band size: 16 kDa; Observed band size: 16 kDa Immunocytochemistry analysis diluted at 1:200 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Butyrly-Hist1H3A (K18) Polyclonal Antibody

BiomatikSKU: CAC11535-100ul
Size: 100ul
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Western Blot; Detected samples: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h; All lanes: HIST1H3A antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/40000 dilution; Predicted band size: 16 kDa; Observed band size: 16 kDa
Immunocytochemistry analysis diluted at 1:200 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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