Atp6V1G2 Polyclonal Antibody, Cat#CAC08253
Immunofluorescence staining of A549 cells with CAC08253 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western blot; ,All lanes: ATP6V1G2 antibody at 12µg/ml; ,Lane 1: Rat heart tissue; ,Lane 2: Mouse spleen tissue; ,Lane 3: Hela whole cell lysate; ,Lane 4: HepG2 whole cell lysate; ,Lane 5: A549 whole cell lysate; ,Lane 6: HT29 whole cell lysate; ,Lane 7: K562 whole cell lysate; ,Secondary; ,Goat polyclonal to rabbit IgG at 1/10000 dilution; ,Predicted band size: 14, 9 kDa; ,Observed band size: 14 kDa
Immunohistochemistry of paraffin-embedded human brain tissue using CAC08253 at dilution of 1:100
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Atp6V1G2 Polyclonal Antibody, Cat#CAC08253 Immunofluorescence staining of A549 cells with CAC08253 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Western blot; ,All lanes: ATP6V1G2 antibody at 12µg/ml; ,Lane 1: Rat heart tissue; ,Lane 2: Mouse spleen tissue; ,Lane 3: Hela whole cell lysate; ,Lane 4: HepG2 whole cell lysate; ,Lane 5: A549 whole cell lysate; ,Lane 6: HT29 whole cell lysate; ,Lane 7: K562 whole cell lysate; ,Secondary; ,Goat polyclonal to rabbit IgG at 1/10000 dilution; ,Predicted band size: 14, 9 kDa; ,Observed band size: 14 kDa Immunohistochemistry of paraffin-embedded human brain tissue using CAC08253 at dilution of 1:100

Atp6V1G2 Polyclonal Antibody

BiomatikSKU: CAC08253-100ug
Size: 100ug
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Immunofluorescence staining of A549 cells with CAC08253 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western blot; ,All lanes: ATP6V1G2 antibody at 12µg/ml; ,Lane 1: Rat heart tissue; ,Lane 2: Mouse spleen tissue; ,Lane 3: Hela whole cell lysate; ,Lane 4: HepG2 whole cell lysate; ,Lane 5: A549 whole cell lysate; ,Lane 6: HT29 whole cell lysate; ,Lane 7: K562 whole cell lysate; ,Secondary; ,Goat polyclonal to rabbit IgG at 1/10000 dilution; ,Predicted band size: 14, 9 kDa; ,Observed band size: 14 kDa
Immunohistochemistry of paraffin-embedded human brain tissue using CAC08253 at dilution of 1:100

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