ATP2B3 Polyclonal Antibody

BiomatikSKU: CAC15746-100ul

Size: 100ul
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Description

Technical Documents

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Western Blot, Positive WB detected in: Jurkat whole cell lysate, All lanes: ATP2B3 antibody at 1:500, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 135, 129, 128, 133, 127, 125, 123 kDa, Observed band size: 135 kDa
Immunofluorescence staining of Hela cells with CAC15746 at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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