Western blot; All lanes: ARFGAP3 antibody at 4µg/ml; Lane 1: HepG2 whole cell lysate; Lane 2: Jurkat whole cell lysate; Lane 3: A549 whole cell lysate; Secondary; Goat polyclonal to rabbit IgG at 1/10000 dilution; Predicted band size: 57, 52 kDa; Observed band size: 57 kDa;
Immunohistochemistry of paraffin-embedded human breast cancer using CAC10669 at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver cancer using CAC10669 at dilution of 1:100
Immunoprecipitating ARFGAP3 in HepG2 whole cell lysate; , Lane 1: Rabbit control IgG instead of (1µg) instead in HepG2 whole cell lysate., For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000); , Lane 2: CAC10669 (4µg) + HepG2 whole cell lysate (500µg); , Lane 3: HepG2 whole cell lysate (20µg);