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Western blot; All lanes: APEX1 antibody at 2µg/ml; Lane 1: Hela whole cell lysate; Lane 2: Mouse brain tissue; Lane 3: MCF-7 whole cell lysate; Lane 4: A431 whole cell lysate; Secondary; Goat polyclonal to rabbit IgG at 1/15000 dilution; Predicted band size: 36 kDa; Observed band size: 36 kDa
Immunohistochemistry of paraffin-embedded human cervical cancer using CAC07408 at dilution of 1:100
Chromatin Immunoprecipitation MCF-7 (1.1*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4µg anti-APEX1 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the MDR1 promoter.

Safety Data Sheet