Ultra Sensitive IHC

Anxa5 Polyclonal Antibody

BiomatikSKU: CAC07114-100ug

Size: 100ug
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Description

Technical Documents

Images

Western Blot; Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate, A549 whole cell lysate; All lanes: ANXA5 antibody at 1:3000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 36 kDa; Observed band size: 36 kDa
IHC image diluted at 1:500 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image diluted at 1:500 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry of paraffin-embedded human placenta tissue using CAC07114 at dilution of 1:100

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