Western blot; All lanes: Angiomotin antibody at 2µg/ml + 293T whole cell lysate; Secondary; Goat polyclonal to rabbit IgG at 1/10000 dilution; Predicted band size: 119, 73 kDa; Observed band size: 119 kDa;
Western Blot; ,Positive WB detected in: A549 whole cell lysate; ,All lanes: AMOT antibody at 1.21µg/ml; ,Secondary; ,Goat polyclonal to rabbit IgG at 1/50000 dilution; ,Predicted band size: 119 kDa; ,Observed band size: 130 kDa;
IHC image diluted at 1:121 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image diluted at 1:121 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.