Akt2 Polyclonal Antibody

BiomatikSKU: CAC09191-100ug

Size: 100ug
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Description

Technical Documents

Images

Western Blot; Positive WB detected in: MCF-7 whole cell lysate, Rat liver tissue; All lanes: AKT2 antibody at 2.8µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 56, 52 kDa; Observed band size: 56 kDa
Immunohistochemistry of paraffin-embedded human lung cancer using CAC09191 at dilution of 1:100
Immunofluorescence staining of A549 cells with CAC09191 at 1:466, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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