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Immunohistochemistry of paraffin-embedded human kidney tissue using CAC07145 at dilution of 1:100
IHC image diluted at 1:1200 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image diluted at 1:1200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western blot; , All lanes: Protein argonaute-2 antibody at 2µg/ml; , Lane 1: A549 whole cell lysate; , Lane 2: Jurkats whole cell lysate; , Lane 3: MCF-7 whole cell lysate; , Lane 4: HepG2 whole cell lysate; , Lane 5: Raw264.7 whole cell lysate; , Lane 6: K562 whole cell lysate; , Lane 7: Mouse liver tissue; , Lane 8: Mouse kidney tissue; , Secondary; , Goat polyclonal to rabbit IgG at 1/10000 dilution; , Predicted band size: 98, 94 kDa; , Observed band size: 94 kDa

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