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Western Blot, Positive WB detected in: HepG2 whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, All lanes: ADRA1A antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 52, 53, 48, 51, 33, 38, 36, 41 kDa, Observed band size: 52 kDa
Immunofluorescence staining of HepG2 cells with CAC15719 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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