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Technical Documents
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Western blot; All lanes: ADH1B antibody at 2µg/ml + HepG2 whole cell lysate; Secondary; Goat polyclonal to rabbit IgG at 1/15000 dilution; Predicted band size: 40, 36 kDa; Observed band size: 40 kDa
Immunohistochemistry of paraffin-embedded human liver tissue using CAC08211 at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver cancer using CAC08211 at dilution of 1:100
Immunofluorescence staining of HepG2 cells with CAC08211 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Safety Data Sheet