Actn1 Polyclonal Antibody

BiomatikSKU: CAC11647-100ul

Size: 100ul
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Technical Documents

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Western Blot; Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, PC3 whole cell lysate, U251 whole cell lysate, U87 whole cell lysate; All lanes: ACTN1 antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 104, 103, 106, 108 kDa; Observed band size: 104 kDa;
Immunofluorescence staining of Hela cells with CAC11647 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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