Acetyl-HIST1H4A (K16) Polyclonal Antibody

BiomatikSKU: CAC15226-100ul

Size: 100ul
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Technical Documents

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Western Blot, Positive WB detected in: Hela cell acid extracts treated by 15mM NaB for 60min, All lanes: HIST1H4A antibody at 1.6ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 12 kDa, Observed band size: 12 kDa
Immunocytochemistry analysis of Hela cells using CAC15226 at dilution of 1:100
Immunofluorescent analysis of Hela cells using CAC15226 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Chromatin Immunoprecipitation Hela (4*10 6 , treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8ug anti-HIST1H4A (CAC15226) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

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