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Western Blot, Detected samples: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h, All lanes: HIST1H3A antibody at 1:500, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 16 kDa, Observed band size: 16 kDa
Immunocytochemistry analysis of Hela cells using CAC15164 at dilution of 1:100
Chromatin Immunoprecipitation Hela (4*10 6 , treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H3A (CAC15164) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

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