Acetyl-HIST1H2BC (K24) Polyclonal Antibody, Cat#CAC15596
Western Blot, Detected samples: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h, All lanes: HIST1H2BC antibody at 1:100, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 14 kDa, Observed band size: 14 kDa
Immunocytochemistry analysis of CAC15596 diluted at 1:10 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating HIST1H2BC in A549 whole cell lysate, Lane 1: Rabbit control IgG instead of CAC15596 in A549 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC15596 (5ug) + A549 whole cell lysate (500ug), Lane 3: A549 whole cell lysate (20ug)
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Acetyl-HIST1H2BC (K24) Polyclonal Antibody, Cat#CAC15596 Western Blot, Detected samples: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h, All lanes: HIST1H2BC antibody at 1:100, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 14 kDa, Observed band size: 14 kDa Immunocytochemistry analysis of CAC15596 diluted at 1:10 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunoprecipitating HIST1H2BC in A549 whole cell lysate, Lane 1: Rabbit control IgG instead of CAC15596 in A549 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC15596 (5ug) + A549 whole cell lysate (500ug), Lane 3: A549 whole cell lysate (20ug)

Acetyl-HIST1H2BC (K24) Polyclonal Antibody

BiomatikSKU: CAC15596-100ul
Size: 100ul
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Western Blot, Detected samples: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h, All lanes: HIST1H2BC antibody at 1:100, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 14 kDa, Observed band size: 14 kDa
Immunocytochemistry analysis of CAC15596 diluted at 1:10 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating HIST1H2BC in A549 whole cell lysate, Lane 1: Rabbit control IgG instead of CAC15596 in A549 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC15596 (5ug) + A549 whole cell lysate (500ug), Lane 3: A549 whole cell lysate (20ug)

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