Acetyl-HIST1H2BC (K15) Polyclonal Antibody, Cat#CAC15630
Western Blot, Detected samples: Hela whole cell lysate, K562 whole cell lysate, 293 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h, All lanes: HIST1H2BC antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 14 kDa, Observed band size: 14 kDa
Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CAC15630 at 1:5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating HIST1H2BC in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h), Lane 1: Rabbit control IgG instead of CAC15630 in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h). For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC15630 (5ug) + HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (500ug), Lane 3: HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (20ug)
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Acetyl-HIST1H2BC (K15) Polyclonal Antibody, Cat#CAC15630 Western Blot, Detected samples: Hela whole cell lysate, K562 whole cell lysate, 293 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h, All lanes: HIST1H2BC antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 14 kDa, Observed band size: 14 kDa Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CAC15630 at 1:5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunoprecipitating HIST1H2BC in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h), Lane 1: Rabbit control IgG instead of CAC15630 in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h). For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC15630 (5ug) + HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (500ug), Lane 3: HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (20ug)

Acetyl-HIST1H2BC (K15) Polyclonal Antibody

BiomatikSKU: CAC15630-100ul
Size: 100ul
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Western Blot, Detected samples: Hela whole cell lysate, K562 whole cell lysate, 293 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h, All lanes: HIST1H2BC antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 14 kDa, Observed band size: 14 kDa
Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CAC15630 at 1:5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating HIST1H2BC in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h), Lane 1: Rabbit control IgG instead of CAC15630 in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h). For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC15630 (5ug) + HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (500ug), Lane 3: HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (20ug)

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