Acetyl-Hist1H2Bc (K11) Polyclonal Antibody, Cat#CAC11527
Western Blot; Detected samples: 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h; All lanes: HIST1H2BC antibody at 1:100; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 14 kDa; Observed band size: 14 kDa
Immunocytochemistry analysis diluted at 1:20 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Acetyl-Hist1H2Bc (K11) Polyclonal Antibody, Cat#CAC11527 Western Blot; Detected samples: 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h; All lanes: HIST1H2BC antibody at 1:100; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 14 kDa; Observed band size: 14 kDa Immunocytochemistry analysis diluted at 1:20 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Acetyl-Hist1H2Bc (K11) Polyclonal Antibody

BiomatikSKU: CAC11527-100ul
Size: 100ul
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Western Blot; Detected samples: 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h; All lanes: HIST1H2BC antibody at 1:100; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 14 kDa; Observed band size: 14 kDa
Immunocytochemistry analysis diluted at 1:20 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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