Acetyl-Hist1H2Ag (K36) Polyclonal Antibody, Cat#CAC11300
Immunocytochemistry analysis of Hela cells using CAC11300 at dilution of 1:100
Immunofluorescent analysis of Hela cells (sodium butyrate, 30 mM, 4h) using CAC11300 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H2AG (CAC11300) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ß-Globin promoter.
Western Blot; , Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate (All treated with 30mM sodium butyrate for 4h); , All lanes: HIST1H2AG antibody at 1µg/ml; , Secondary; , Goat polyclonal to rabbit IgG at 1/50000 dilution; , Predicted band size: 15 kDa; , Observed band size: 15 kDa
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Acetyl-Hist1H2Ag (K36) Polyclonal Antibody, Cat#CAC11300 Immunocytochemistry analysis of Hela cells using CAC11300 at dilution of 1:100 Immunofluorescent analysis of Hela cells (sodium butyrate, 30 mM, 4h) using CAC11300 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H2AG (CAC11300) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ß-Globin promoter. Western Blot; , Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate (All treated with 30mM sodium butyrate for 4h); , All lanes: HIST1H2AG antibody at 1µg/ml; , Secondary; , Goat polyclonal to rabbit IgG at 1/50000 dilution; , Predicted band size: 15 kDa; , Observed band size: 15 kDa

Acetyl-Hist1H2Ag (K36) Polyclonal Antibody

BiomatikSKU: CAC11300-100ul
Size: 100ul
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Immunocytochemistry analysis of Hela cells using CAC11300 at dilution of 1:100
Immunofluorescent analysis of Hela cells (sodium butyrate, 30 mM, 4h) using CAC11300 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H2AG (CAC11300) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ß-Globin promoter.
Western Blot; , Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate (All treated with 30mM sodium butyrate for 4h); , All lanes: HIST1H2AG antibody at 1µg/ml; , Secondary; , Goat polyclonal to rabbit IgG at 1/50000 dilution; , Predicted band size: 15 kDa; , Observed band size: 15 kDa

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