Acetyl-HIST1H1C (K74) Polyclonal Antibody

BiomatikSKU: CAC15677-100ul

Size: 100ul
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Technical Documents

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Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CAC15677 at 1:7.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Chromatin Immunoprecipitation Hela (10 6 , treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H1C (CAC15677) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

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