Acetyl-H2AFZ (K4) Polyclonal Antibody, Cat#CAC12853
Western Blot, Positive WB detected in: K562 whole cell lysate (treated by 30mM sodium butyrate for 4h), All lanes: H2AFZ antibody at 1.06ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 14 kDa, Observed band size: 14 kDa
Immunofluorescence staining of Hela cells with CAC12853 at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Chromatin Immunoprecipitation Hela (4*10 6 , treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-H2AFZ (CAC12853) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
Immunocytochemistry analysis of CAC12853 diluted at 1:20 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Acetyl-H2AFZ (K4) Polyclonal Antibody, Cat#CAC12853 Western Blot, Positive WB detected in: K562 whole cell lysate (treated by 30mM sodium butyrate for 4h), All lanes: H2AFZ antibody at 1.06ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 14 kDa, Observed band size: 14 kDa Immunofluorescence staining of Hela cells with CAC12853 at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Chromatin Immunoprecipitation Hela (4*10 6 , treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-H2AFZ (CAC12853) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter. Immunocytochemistry analysis of CAC12853 diluted at 1:20 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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Acetyl-H2AFZ (K4) Polyclonal Antibody

BiomatikSKU: CAC12853-100ul
Size: 100ul
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Western Blot, Positive WB detected in: K562 whole cell lysate (treated by 30mM sodium butyrate for 4h), All lanes: H2AFZ antibody at 1.06ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 14 kDa, Observed band size: 14 kDa
Immunofluorescence staining of Hela cells with CAC12853 at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Chromatin Immunoprecipitation Hela (4*10 6 , treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-H2AFZ (CAC12853) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
Immunocytochemistry analysis of CAC12853 diluted at 1:20 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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We were very pleased with Biomatik’s performance on a custom recombinant protein project earlier this summer. Communication throughout the project was excellent, technical questions were addressed promptly, and the supporting analytical and QC data were clear and comprehensive. The recombinant protein was produced to the requested specifications and delivered on schedule. Based on this experience, we have chosen Biomatik for an additional custom protein production project and would be pleased to work with them again.

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We’ve been using Biomtik for peptide synthesis in our research, and they have proven to be extremely reliable. Turnaround times are consistent, product quality is excellent, and communication is always clear and professional. Highly recommend their services to any lab looking for dependable peptide synthesis.

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Biomatik provided excellent support to our Biometrology team at NPL, with timely and reliable protein production services. The quality of the material delivered was to the expected standards, and the communication throughout the project was smooth and efficient.

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We got help from Biomatik for our studies, and the experience was excellent. The customer support team was responsive and knowledgeable, providing detailed documentation and timely updates throughout the process. We appreciate the reliable quality and professional service, and we look forward to working with Biomatik again for further.

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I work on molecular farming, producing recombinant protein in plant suspention cells. I'm completely satisfied after almost 5 years ordering antibidies at Biomatik. Perfect reproducibility batch after batch and I hope to continiue like this!

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Yue Home Liu

We have used Biomatik to generate two different antibodies and in both cases they worked extremely well in all the assays we tested, including ELISA, WB, and IFA. I would recommend this company.

Sarah Wootton

The peptide product is of exceptional quality and is highly recommended for its efficacy.

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I have been using Biomatik's modified peptides for a while and they deliver high quality peptides and a very good technical support

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I have bought peptides from Biomatic several times and the quality is the best!!!

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