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Immunofluorescent analysis of Hela cells (sodium butyrate, 30 mM, 4h) using CAC15251 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Chromatin Immunoprecipitation Hela (4*10 6 , treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8ug anti-H2AFZ (CAC15251) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
Western Blot, Positive WB detected in: HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h), All lanes: H2AFZ antibody at 1ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 14 kDa, Observed band size: 14 kDa

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