Acaa2 Polyclonal Antibody, Cat#CAC11123
Western Blot; Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, NIH/3T3 whole cell lysate, HepG2 whole cell lysate, Rat lung tissue, Mouse liver tissue, Mouse lung tissue; All lanes: ACAA2 antibody at 2.4µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 42 kDa; Observed band size: 42 kDa
IHC image diluted at 1:700 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescent analysis of HepG2 cells using CAC11123 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunoprecipitating ACAA2 in A549 whole cell lysate ; , Lane 1: Rabbit control IgG (1ug) instead in A549 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: CAC11123 (6ug) + A549 whole cell lysate (500ug); , Lane 3: A549 whole cell lysate (10ug)
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Acaa2 Polyclonal Antibody, Cat#CAC11123 Western Blot; Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, NIH/3T3 whole cell lysate, HepG2 whole cell lysate, Rat lung tissue, Mouse liver tissue, Mouse lung tissue; All lanes: ACAA2 antibody at 2.4µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 42 kDa; Observed band size: 42 kDa IHC image diluted at 1:700 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescent analysis of HepG2 cells using CAC11123 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) Immunoprecipitating ACAA2 in A549 whole cell lysate ; , Lane 1: Rabbit control IgG (1ug) instead in A549 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: CAC11123 (6ug) + A549 whole cell lysate (500ug); , Lane 3: A549 whole cell lysate (10ug)

Ultra Sensitive IHC

Acaa2 Polyclonal Antibody

BiomatikSKU: CAC11123-100ug
Size: 100ug
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Western Blot; Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, NIH/3T3 whole cell lysate, HepG2 whole cell lysate, Rat lung tissue, Mouse liver tissue, Mouse lung tissue; All lanes: ACAA2 antibody at 2.4µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 42 kDa; Observed band size: 42 kDa
IHC image diluted at 1:700 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescent analysis of HepG2 cells using CAC11123 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunoprecipitating ACAA2 in A549 whole cell lysate ; , Lane 1: Rabbit control IgG (1ug) instead in A549 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: CAC11123 (6ug) + A549 whole cell lysate (500ug); , Lane 3: A549 whole cell lysate (10ug)

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