2-hydroxyisobutyryl-HIST1H4A (K16) Polyclonal Antibody, Cat#CAC15465
Western Blot, Detected samples: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h, All lanes: HIST1H4A antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 12 kDa, Observed band size: 12 kDa
Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CAC15465 at 1:5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Chromatin Immunoprecipitation Hela (4*10 6 , treated with 30mM sodium butyrate for 4h) were treated with Benzanase, sonicated, and immunoprecipitated with 5ug anti-HIST1H4A (CAC15465) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
Immunoprecipitating HIST1H4A in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h), Lane 1: Rabbit control IgG instead of CAC15465 in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h). For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC15465 (5ug) + HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (500ug), Lane 3: HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (20ug)
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2-hydroxyisobutyryl-HIST1H4A (K16) Polyclonal Antibody, Cat#CAC15465 Western Blot, Detected samples: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h, All lanes: HIST1H4A antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 12 kDa, Observed band size: 12 kDa Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CAC15465 at 1:5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Chromatin Immunoprecipitation Hela (4*10 6 , treated with 30mM sodium butyrate for 4h) were treated with Benzanase, sonicated, and immunoprecipitated with 5ug anti-HIST1H4A (CAC15465) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter. Immunoprecipitating HIST1H4A in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h), Lane 1: Rabbit control IgG instead of CAC15465 in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h). For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC15465 (5ug) + HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (500ug), Lane 3: HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (20ug)

2-hydroxyisobutyryl-HIST1H4A (K16) Polyclonal Antibody

BiomatikSKU: CAC15465-100ul
Size: 100ul
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Western Blot, Detected samples: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h, All lanes: HIST1H4A antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 12 kDa, Observed band size: 12 kDa
Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CAC15465 at 1:5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Chromatin Immunoprecipitation Hela (4*10 6 , treated with 30mM sodium butyrate for 4h) were treated with Benzanase, sonicated, and immunoprecipitated with 5ug anti-HIST1H4A (CAC15465) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
Immunoprecipitating HIST1H4A in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h), Lane 1: Rabbit control IgG instead of CAC15465 in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h). For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC15465 (5ug) + HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (500ug), Lane 3: HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (20ug)

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