2-hydroxyisobutyryl-HIST1H4A (K12) Polyclonal Antibody, Cat#CAC15715
Western Blot, Detected samples: Hela whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h, All lanes: HIST1H4A antibody at 1.25ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 12 kDa, Observed band size: 12 kDa
Immunocytochemistry analysis of CAC15715 diluted at 1:30 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CAC15715 at 1:15, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating HIST1H4A in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h), Lane 1: Rabbit control IgG instead of CAC15715 in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h). For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC15715 (3ug) + HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (500ug), Lane 3: HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (20ug)
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2-hydroxyisobutyryl-HIST1H4A (K12) Polyclonal Antibody, Cat#CAC15715 Western Blot, Detected samples: Hela whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h, All lanes: HIST1H4A antibody at 1.25ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 12 kDa, Observed band size: 12 kDa Immunocytochemistry analysis of CAC15715 diluted at 1:30 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CAC15715 at 1:15, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunoprecipitating HIST1H4A in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h), Lane 1: Rabbit control IgG instead of CAC15715 in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h). For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC15715 (3ug) + HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (500ug), Lane 3: HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (20ug)

2-hydroxyisobutyryl-HIST1H4A (K12) Polyclonal Antibody

BiomatikSKU: CAC15715-100ul
Size: 100ul
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Western Blot, Detected samples: Hela whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h, All lanes: HIST1H4A antibody at 1.25ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 12 kDa, Observed band size: 12 kDa
Immunocytochemistry analysis of CAC15715 diluted at 1:30 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CAC15715 at 1:15, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating HIST1H4A in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h), Lane 1: Rabbit control IgG instead of CAC15715 in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h). For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC15715 (3ug) + HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (500ug), Lane 3: HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (20ug)

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