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Western Blot,, Positive WB detected in:MCF7 whole cell lysate (30ug), HEK293 whole cell lysate (30ug), U251 whole cell lysate (30ug), COLO205 whole cell lysate (30ug), Mouse brainr tissue lysate (30ug), Rat brain lysate (30ug), All lanes: ACVR1B antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 57 kDa, Observed band size: 57 kDa, Exposure time:30s
IHC image of RAC09031 diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of A549 cell with RAC09031 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Fluorescein Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

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