ACTR2 Recombinant Monoclonal Antibody, Cat#RAC09000
Western Blot,, Positive WB detected in:Jurkat whole cell lysate (30ug), A549 whole cell lysate (30ug), THP-1 whole cell lysate (30ug), HepG2 whole cell lysate (30ug), MCF7 whole cell lysate (30ug), SH-SY5Y whole cell lysate (30ug), Mouse brain tissue lysate (30ug), Mouse spleen tissue lysate (30ug), Rat kidney lysate (30ug), All lanes: Actin related protein 2 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 45 kDa, Observed band size: 45 kDa, Exposure time:10s
Overlay Peak curve showing jurkat cells stained with RAC09000 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10_6cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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ACTR2 Recombinant Monoclonal Antibody, Cat#RAC09000 Western Blot,, Positive WB detected in:Jurkat whole cell lysate (30ug), A549 whole cell lysate (30ug), THP-1 whole cell lysate (30ug), HepG2 whole cell lysate (30ug), MCF7 whole cell lysate (30ug), SH-SY5Y whole cell lysate (30ug), Mouse brain tissue lysate (30ug), Mouse spleen tissue lysate (30ug), Rat kidney lysate (30ug), All lanes: Actin related protein 2 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 45 kDa, Observed band size: 45 kDa, Exposure time:10s Overlay Peak curve showing jurkat cells stained with RAC09000 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10_6cells) used under the same conditions. Acquisition of >10, 000 events was performed.

ACTR2 Recombinant Monoclonal Antibody

BiomatikSKU: RAC09000-100ul
Size: 100ul
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Western Blot,, Positive WB detected in:Jurkat whole cell lysate (30ug), A549 whole cell lysate (30ug), THP-1 whole cell lysate (30ug), HepG2 whole cell lysate (30ug), MCF7 whole cell lysate (30ug), SH-SY5Y whole cell lysate (30ug), Mouse brain tissue lysate (30ug), Mouse spleen tissue lysate (30ug), Rat kidney lysate (30ug), All lanes: Actin related protein 2 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 45 kDa, Observed band size: 45 kDa, Exposure time:10s
Overlay Peak curve showing jurkat cells stained with RAC09000 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10_6cells) used under the same conditions. Acquisition of >10, 000 events was performed.

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