RAF1 Polyclonal Antibody, Cat#CAC13895
IHC image of CAC13895 diluted at 1:1200 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CAC13895 at 1:400, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating RAF1 in 293T whole cell lysate, Lane 1: Rabbit control IgG (1ug) instead of CAC13895 in 293T whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC13895 (6ug) + 293T whole cell lysate (500ug), Lane 3: 293T whole cell lysate (10ug)
Western Blot, Positive WB detected in: MCF-7 whole cell lysate, All lanes: RAF1 antibody at 4ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 74, 76 kDa, Observed band size: 74 kDa
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RAF1 Polyclonal Antibody, Cat#CAC13895 IHC image of CAC13895 diluted at 1:1200 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CAC13895 at 1:400, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunoprecipitating RAF1 in 293T whole cell lysate, Lane 1: Rabbit control IgG (1ug) instead of CAC13895 in 293T whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC13895 (6ug) + 293T whole cell lysate (500ug), Lane 3: 293T whole cell lysate (10ug) Western Blot, Positive WB detected in: MCF-7 whole cell lysate, All lanes: RAF1 antibody at 4ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 74, 76 kDa, Observed band size: 74 kDa

Ultra Sensitive IHC

RAF1 Polyclonal Antibody

BiomatikSKU: CAC13895-100ug
Size: 100ug
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IHC image of CAC13895 diluted at 1:1200 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CAC13895 at 1:400, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating RAF1 in 293T whole cell lysate, Lane 1: Rabbit control IgG (1ug) instead of CAC13895 in 293T whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC13895 (6ug) + 293T whole cell lysate (500ug), Lane 3: 293T whole cell lysate (10ug)
Western Blot, Positive WB detected in: MCF-7 whole cell lysate, All lanes: RAF1 antibody at 4ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 74, 76 kDa, Observed band size: 74 kDa

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