Pold1 Polyclonal Antibody, Cat#CAC11242
Western Blot; Positive WB detected in: Jurkat whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, A549 whole cell lysate; All lanes: POLD1 antibody at 5µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 124 kDa; Observed band size: 124 kDa
IHC image diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating POLD1 in Hela whole cell lysate; , Lane 1: Rabbit control IgG instead in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: CAC11242 (8µg) + Hela whole cell lysate (500µg); , Lane 3: Hela whole cell lysate (10µg)
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Pold1 Polyclonal Antibody, Cat#CAC11242 Western Blot; Positive WB detected in: Jurkat whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, A549 whole cell lysate; All lanes: POLD1 antibody at 5µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 124 kDa; Observed band size: 124 kDa IHC image diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunoprecipitating POLD1 in Hela whole cell lysate; , Lane 1: Rabbit control IgG instead in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: CAC11242 (8µg) + Hela whole cell lysate (500µg); , Lane 3: Hela whole cell lysate (10µg)

Ultra Sensitive IHC

Pold1 Polyclonal Antibody

BiomatikSKU: CAC11242-100ug
Size: 100ug
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Western Blot; Positive WB detected in: Jurkat whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, A549 whole cell lysate; All lanes: POLD1 antibody at 5µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 124 kDa; Observed band size: 124 kDa
IHC image diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating POLD1 in Hela whole cell lysate; , Lane 1: Rabbit control IgG instead in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: CAC11242 (8µg) + Hela whole cell lysate (500µg); , Lane 3: Hela whole cell lysate (10µg)

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