Immunoprecipitating GLE1 in A549 whole cell lysate; Lane 1: Rabbit control IgG instead of CAC10456 in A549 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: CAC10456 (6µg) + A549 whole cell lysate (500µg); Lane 3: A549 whole cell lysate (20µg)
Western Blot; ,Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate; ,All lanes: GLE1 antibody at 2.8µg/ml; ,Secondary; ,Goat polyclonal to rabbit IgG at 1/50000 dilution; ,Predicted band size: 80, 76 kDa; ,Observed band size: 80 kDa
Immunocytochemistry analysis of human melanoma using CAC10456 at dilution of 1:100
Immunocytochemistry analysis of human breast cancer using CAC10456 at dilution of 1:100