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Cat#

RPU60767
List Price:
$624.00

 

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Technical Documents:

Target Name

Multimerin 1

Target Synonyms

ECM; EMILIN4; GPIa; MMRN; Glycoprotein Ia; Elastin microfibril interface located protein 4; Endothelial cell multimerin; Platelet glycoprotein Ia; 155 kDa platelet multimerin

Species

Human (Homo sapiens)

Host

E. coli

Protein Type

Active Protein

Tag

N-Terminal His Tag

Activity

Biologically Active

Applications

Cell Culture, Activity Assays

tid

50ug,624;100ug,873.6;1mg,4056

Human Multimerin 1, Active Protein is a purified Active Protein. Purity: >95% by SDS-PAGE. Host: E. coli. Endotoxin Level: <1.0EU per 1ug, by the LAL method. Species: Human (Homo sapiens). Target Name: Multimerin 1. Accession Number: Q13201. Gene ID: 22915. Expression Region: Ala807~Gly1053. Tag Info: N-terminal His Tag. Theoretical MW: 30kda. Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.. Target Synonyms: ECM; EMILIN4; GPIa; MMRN; Glycoprotein Ia; Elastin microfibril interface located protein 4; Endothelial cell multimerin; Platelet glycoprotein Ia; 155 kDa platelet multimerin Restrictions: For Research Use Only. Not for use in diagnostic procedures.

Lead Time

7-11 Business Days

Expression System

Prokaryotic expression

Accession Number

Q13201

Gene ID

22915

Expression Region

Ala807~Gly1053

Theoretical MW

30kDa

Conjugate

Unconjugated

Endotoxin Level

<1.0EU/ug, by the LAL method

Isoelectric Point

9.4

Applications

Cell Culture, Activity Assays

Purity

>95% by SDS-PAGE

Format

Freeze-dried powder

Storage

Store at 2-8°C for one month,-80°C for 12 months

Buffer

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Expiration

Stable for 12 months if correctly stored

Reconstitution

Refer to the datasheet/CoA included in the product pouch.

Shipping Condition

Ice packs

Research Area

Hematology

Quality Guarantee

This product carries the Biomatik 100% Quality Guarantee. Please refer to the Terms and Conditions for details.

Restrictions

For Research Use Only. Not for use in diagnostic procedures.

Quality Systems

This product is manufactured at ISO 9001 and ISO 13485 certified facilities.

Technical Documents

Selected Citations Featuring Biomatik's Products & Services
Last updated 2020-01-31

 

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SDS-PAGE
Multimerin 1 (MMRN1) is a secreted glycoprotein that consists of multiple subunits, forming a complex with a molecular weight of approximately 150 kDa. MMRN1 is a member of the multimerin family and it is mainly expressed in platelets and endothelial cells. MMRN1 plays an important role in maintaining vascular integrity, regulating clotting and inflammatory responses, among others. In addition, the binding of MMRN1 to Vitronectin (VTN) plays an important role in a variety of physiological and pathological processes, including cell adhesion, migration, blood coagulation, and cell signaling. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human MMRN1 and recombinant human VTN. Briefly, MMRN1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to VTN-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-MMRN1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37°C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human MMRN1 and recombinant human VTN was shown, and this effect was in a dose dependent manner.

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