Trusted by 10,000+ Scientists Since 2002. View Our 5-Star Google Review, Select Citations and 4,000+ Citations at Google Scholar.

Cat#

RPU60581
List Price:
$587.50

Attention US customers:
For offline orders, please request a quote before purchasing.

 

Quantity:

Request Information
Technical Documents:

Target Name

Acid Phosphatase 6, Lysophosphatidic

Target Synonyms

LPAP; ACPL1; PACPL1; Acid phosphatase-like protein 1; Lysophosphatidic acid phosphatase type 6

Species

Human (Homo sapiens)

Host

E. coli

Protein Type

Active Protein

Tag

N-Terminal His Tag

Activity

Biologically Active

Applications

Cell Culture, Activity Assays

Human Acid Phosphatase 6, Lysophosphatidic, Active Protein is a purified Active Protein. Purity: >90% by SDS-PAGE. Host: E. coli. Endotoxin Level: <1.0EU per 1ug, by the LAL method. Species: Human (Homo sapiens). Target Name: Acid Phosphatase 6, Lysophosphatidic. Accession Number: Q9NPH0. Gene ID: 51205. Expression Region: Met1~Glu428. Tag Info: N-terminal His Tag. Theoretical MW: 53kda. Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.. Target Synonyms: LPAP; ACPL1; PACPL1; Acid phosphatase-like protein 1; Lysophosphatidic acid phosphatase type 6 Restrictions: For Research Use Only. Not for use in diagnostic procedures.

Lead Time

7-11 Business Days

Expression System

Prokaryotic expression

Accession Number

Q9NPH0

Gene ID

51205

Expression Region

Met1~Glu428

Theoretical MW

53kDa

Conjugate

Unconjugated

Endotoxin Level

<1.0EU/ug, by the LAL method

Isoelectric Point

6

Applications

Cell Culture, Activity Assays

Purity

>90% by SDS-PAGE

Format

Freeze-dried powder

Storage

Store at 2-8°C for one month,-80°C for 12 months

Buffer

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Expiration

Stable for 12 months if correctly stored

Reconstitution

Refer to the datasheet/CoA included in the product pouch.

Shipping Condition

Ice packs

Research Area

Enzyme, Kinase

Quality Guarantee

This product carries the Biomatik 100% Quality Guarantee. Please refer to the Terms and Conditions for details.

Restrictions

For Research Use Only. Not for use in diagnostic procedures.

Quality Systems

This product is manufactured at ISO 9001 and ISO 13485 certified facilities.

Technical Documents

Selected Citations Featuring Biomatik's Products & Services
Last updated 2020-01-31

 

 Click here to view more selected publications citing Biomatik's fine products & services. .

 Click Google Scholar to view 3,000+ publications citing Biomatik's fine products & services. .

SDS-PAGE
Lysophosphatidic acid phosphatase type 6 (ACP6), is a 44-47 kDa monomeric member of the histidine acid phosphatase family of proteins. ACP6 is highly expressed in kidney, heart, small intestine, muscle, liver, prostate, testis, ovary and exists as two isoforms as a result of alternative splicing events. It plays a role in regulating lipid metabolism that hydrolyzes LPA to monoacylglycerol (MAG) and phosphate. Annexin V (ANXA5) binds to phospholipid bilayers, forming two-dimensional crystals that block the phospholipids from availability for coagulation enzyme reactions. A functional binding ELISA assay was conducted to detect the interaction of recombinant human ACP6 and recombinant mouse ANXA5. Briefly, ACP6 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to ANXA5-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-ACP6 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37°C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human ACP6 and recombinant mouse ANXA5 was shown, the EC50 for this effect is 0.08 ug/mL.

To edit this page simply login to the control panel, click the Website Content tab and choose the View Web Pages option. Click Edit next to the Shipping & Returns page and you can change this text. A sample returns policy is shown below which you can edit as needed.

Returns Policy

You may return most new, unopened items within 30 days of delivery for a full refund. We'll also pay the return shipping costs if the return is a result of our error (you received an incorrect or defective item, etc.).

You should expect to receive your refund within four weeks of giving your package to the return shipper, however, in many cases you will receive a refund more quickly. This time period includes the transit time for us to receive your return from the shipper (5 to 10 business days), the time it takes us to process your return once we receive it (3 to 5 business days), and the time it takes your bank to process our refund request (5 to 10 business days).

If you need to return an item, simply login to your account, view the order using the "Complete Orders" link under the My Account menu and click the Return Item(s) button. We'll notify you via e-mail of your refund once we've received and processed the returned item.

Shipping

We can ship to virtually any address in the world. Note that there are restrictions on some products, and some products cannot be shipped to international destinations.

When you place an order, we will estimate shipping and delivery dates for you based on the availability of your items and the shipping options you choose. Depending on the shipping provider you choose, shipping date estimates may appear on the shipping quotes page.

Please also note that the shipping rates for many items we sell are weight-based. The weight of any such item can be found on its detail page. To reflect the policies of the shipping companies we use, all weights will be rounded up to the next full pound.

Integer et est tellus non bibendum est. Namcos tempus turpis at metus scelerisque placerat nulla eu sollicitudin felis. Pellentesque diam dolor elementum et lobortis at mollis ut risus. Sed faucibus ullamcorper mattis. Fusce molestie elit a loremos tempus scelerisque blandit tortor cursus. Quisque dolutpat orci ut metus malesuada lorem in interdum lectus scelerisque. Praesent eu odio ut nisi ullamcorper ultricies. Cum sociis natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus.

Praesent at justo congue leo adipiscing
  • Integer et est tellusInteger et est tellus non bibendum est.
  • Namcos tempusNamcos tempus turpis at metus scelerisque placerat nulla eu sollicitudin felis.
  • Pellentesque diam dolorPellentesque diam dolor elementum et lobortis at mollis ut risus.
  • Sed faucibusSed faucibus ullamcorper mattis.
  • Fusce molestie elitosFusce molestie elit a loremos tempus scelerisque blandit tortor cursus.
  • Quisque dolutpat orcisQuisque dolutpat orci ut metus malesuada lorem in interdum lectus scelerisque.
  • Praesent an modioPraesent eu odio ut nisi ullamcorper ultricies.
  • Cum sociis natoque penatibusCum sociis natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus.

Subscribe to Receive Updates & Promotions from Biomatik

Top