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Cat#

CAC12347
List Price:
$252.00

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Target Name

MAS1L

Target Synonyms

Mas-related G-protein coupled receptor MRG (MAS-R) (MAS1-like); MAS1L; MRG

Species

Human (Homo sapiens)

Species Reactivity

Human (Homo sapiens), Rat (Rattus norvegicus)

Antibody Type

Recombinant Antibody

Host

HEK293F Cell

Isotype

Rabbit IgG

Clonality

Monoclonal

Immunogen

A Synthesized Peptide Derived From Human Mas1L

Applications

ELISA, WB, IF, FC

Mas1L Recombinant Antibody is a Human (Homo sapiens) Recombinant Antibody to MAS1L. The Mas1L Recombinant Antibody has been validated for the following applications: ELISA, WB, IF, FC. Host: HEK293F Cell. Species: Human (Homo sapiens). Immunogen: A Synthesized Peptide Derived From Human Mas1L. Applications: ELISA, WB, IF, FC. Target Synonyms: MAS1L. Accession Number: P35410. Storage: 2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles. Restrictions: For Research Use Only. Not for use in diagnostic procedures.

Mas1L Recombinant Antibody is a Human (Homo sapiens) Recombinant Antibody to MAS1L. The Mas1L Recombinant Antibody has been validated for the following applications: ELISA, WB, IF, FC.

Lead Time

7-11 Business Days

Accession Number

P35410

Clone Number

15B8

Conjugate

Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.

Applications

ELISA, WB, IF, FC

Purification

Affinity-Chromatography

Format

Liquid

Storage

2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.

Buffer

Rabbit IgG in Phosphate Buffered Saline, pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol

Expiration

12 months

Shipping Condition

Ice packs

Research Area

Neuroscience, Signal Transduction

Quality Guarantee

This product carries the Biomatik 100% Quality Guarantee. Please refer to the Terms and Conditions for details.

Restrictions

For Research Use Only. Not for use in diagnostic procedures.

Quality Systems

This product is manufactured at ISO 9001 certified facilities.

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Western Blot; Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, Jurkat whole cell lysate, Rat liver tissue; All lanes: MAS1L antibody at 1:1000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 43 kDa; Observed band size: 36-50 kDa
Immunofluorescence staining of MCF-7 cell with CAC12347 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 595-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of Hela cell with CAC12347 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 595-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing Hela cells stained with CAC12347 (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

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